2JZZ
Solid-State NMR Structure of Microcrystalline Ubiquitin
Summary for 2JZZ
| Entry DOI | 10.2210/pdb2jzz/pdb |
| NMR Information | BMRB: 7111 |
| Descriptor | Ubiquitin (1 entity in total) |
| Functional Keywords | protein microcrystal, cytoplasm, ubl conjugation, signaling protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 8576.83 |
| Authors | Manolikas, T.,Herrmann, T.,Meier, B.H. (deposition date: 2008-01-22, release date: 2008-03-25, Last modification date: 2024-05-08) |
| Primary citation | Manolikas, T.,Herrmann, T.,Meier, B.H. Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy. J.Am.Chem.Soc., 130:3959-3966, 2008 Cited by PubMed Abstract: Proton-driven 13C spin diffusion (PDSD) is a simple and robust two-dimensional NMR experiment. It leads to spectra with a high signal-to-noise ratio in which cross-peaks contain information about internuclear distances. We show that the total information content is sufficient to determine the atomic-resolution structure of a small protein from a single, uniformly 13C-, 15N-labeled microcrystalline sample. For the example of ubiquitin, the structure was determined by a manual procedure followed by an automatic optimization of the manual structure as well as by a fully automated structure determination approach. The relationship between internuclear distances and cross-peak intensities in the spectra is investigated. PubMed: 18321098DOI: 10.1021/ja078039s PDB entries with the same primary citation |
| Experimental method | SOLID-STATE NMR |
Structure validation
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