2JZZ

Solid-State NMR Structure of Microcrystalline Ubiquitin

2JZZ の概要

• エントリーDOI: 10.2210/pdb2jzz/pdb
• NMR情報: BMRB: 7111
• 分子名称: Ubiquitin (1 entity in total)
• 機能のキーワード: protein microcrystal, cytoplasm, ubl conjugation, signaling protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8576.83

構造登録者

Manolikas, T.,Herrmann, T.,Meier, B.H. (登録日: 2008-01-22, 公開日: 2008-03-25, 最終更新日: 2024-05-08)

主引用文献

Manolikas, T.,Herrmann, T.,Meier, B.H.
Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy.
J.Am.Chem.Soc., 130:3959-3966, 2008

PubMed Abstract: Proton-driven 13C spin diffusion (PDSD) is a simple and robust two-dimensional NMR experiment. It leads to spectra with a high signal-to-noise ratio in which cross-peaks contain information about internuclear distances. We show that the total information content is sufficient to determine the atomic-resolution structure of a small protein from a single, uniformly 13C-, 15N-labeled microcrystalline sample. For the example of ubiquitin, the structure was determined by a manual procedure followed by an automatic optimization of the manual structure as well as by a fully automated structure determination approach. The relationship between internuclear distances and cross-peak intensities in the spectra is investigated.

PubMed: 18321098
DOI: 10.1021/ja078039s

実験手法

SOLID-STATE NMR