2JZT

Solution NMR structure of Q8ZP25_SALTY from Salmonella typhimurium. Northeast Structural Genomics Consortium target StR70

2JZT の概要

• エントリーDOI: 10.2210/pdb2jzt/pdb
• 分子名称: Putative thiol-disulfide isomerase and thioredoxin (1 entity in total)
• 機能のキーワード: nesg, str70, structural genomics, putative [nife] hydrogenase assembly, chaperone, isomerase, psi-2, protein structure initiative, northeast structural genomics consortium
• 由来する生物種: Salmonella typhimurium LT2
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16146.18

構造登録者

Parish, D.,Liu, G.,Shen, Y.,Ho, C.,Cunningham, K.,Xiao, R.,Swapna, G.V.T.,Acton, T.,Bansal, S.,Prestegard, J.H.,Montelione, G.T.,Szyperski, T.,Northeast Structural Genomics Consortium (NESG) (登録日: 2008-01-16, 公開日: 2008-02-19, 最終更新日: 2024-05-29)

主引用文献

Parish, D.,Benach, J.,Liu, G.,Singarapu, K.K.,Xiao, R.,Acton, T.,Su, M.,Bansal, S.,Prestegard, J.H.,Hunt, J.,Montelione, G.T.,Szyperski, T.
Protein chaperones Q8ZP25_SALTY from Salmonella typhimurium and HYAE_ECOLI from Escherichia coli exhibit thioredoxin-like structures despite lack of canonical thioredoxin active site sequence motif.
J.STRUCT.FUNCT.GENOM., 9:41-49, 2008

PubMed Abstract: The structure of the 142-residue protein Q8ZP25_SALTY encoded in the genome of Salmonella typhimurium LT2 was determined independently by NMR and X-ray crystallography, and the structure of the 140-residue protein HYAE_ECOLI encoded in the genome of Escherichia coli was determined by NMR. The two proteins belong to Pfam (Finn et al. 34:D247-D251, 2006) PF07449, which currently comprises 50 members, and belongs itself to the 'thioredoxin-like clan'. However, protein HYAE_ECOLI and the other proteins of Pfam PF07449 do not contain the canonical Cys-X-X-Cys active site sequence motif of thioredoxin. Protein HYAE_ECOLI was previously classified as a [NiFe] hydrogenase-1 specific chaperone interacting with the twin-arginine translocation (Tat) signal peptide. The structures presented here exhibit the expected thioredoxin-like fold and support the view that members of Pfam family PF07449 specifically interact with Tat signal peptides.

PubMed: 19039680
DOI: 10.1007/s10969-008-9050-y

実験手法

SOLUTION NMR