2JZM

Chymotrypsin inhibitor C1 from Nicotiana alata

2JZM の概要

• エントリーDOI: 10.2210/pdb2jzm/pdb
• 分子名称: Proteinase inhibitor (1 entity in total)
• 機能のキーワード: protein, proteinase inhibitor, chymotrypsin inhibitor, plant, insecticidal, hydrolase inhibitor
• 由来する生物種: Nicotiana alata (Winged tobacco)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5746.48

構造登録者

Schirra, H.J.,Anderson, M.A.,Craik, D.J. (登録日: 2008-01-09, 公開日: 2008-11-25, 最終更新日: 2024-11-20)

主引用文献

Schirra, H.J.,Anderson, M.A.,Craik, D.J.
Structural refinement of insecticidal plant proteinase inhibitors from Nicotiana alata.
Protein Pept.Lett., 15:903-909, 2008

PubMed Abstract: Ornamental tobacco (Nicotiana alata) produces a series of 6 kDa proteinase inhibitors belonging to the potato type II inhibitor family. These proteins inhibit trypsin and chymotrypsin, the main digestive enzymes of predatory insects, thus leading to starvation, impaired larval development or death. In this context, the three-dimensional structures of these inhibitors are important for developing novel strategies for pest control. The solution structures of C1 and T1, the two main prototypes of the N. alata inhibitors, were originally determined more than a decade ago (J. Mol. Biol. 242, 231-243 (1994) and Biochemistry 34, 14304-14311 (1995)). Since then methods for NMR structure calculations have evolved considerably. Here we report the refinement of the structures of C1 and T1 with state-of-the-art protocols for NMR structure calculations. This refinement leads to an improved quality of the structures, making them a more reliable basis for the development of novel pesticides and modeling applications.

PubMed: 18991765
DOI: 10.2174/092986608785849326

実験手法

SOLUTION NMR