2JXW

Solution Structure of the Tandem WW Domains of FBP21

2JXW の概要

• エントリーDOI: 10.2210/pdb2jxw/pdb
• NMR情報: BMRB: 15575
• 分子名称: WW domain-binding protein 4 (1 entity in total)
• 機能のキーワード: ww domain containing protein, ww domain, fbp21, wbp4, metal-binding, mrna processing, mrna splicing, nucleus, polymorphism, spliceosome, zinc, zinc-finger, formin binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8688.31

構造登録者

Huang, X.,Zhang, J.,Wu, J.,Shi, Y. (登録日: 2007-11-30, 公開日: 2008-12-02, 最終更新日: 2024-05-15)

主引用文献

Huang, X.,Beullens, M.,Zhang, J.,Zhou, Y.,Nicolaescu, E.,Lesage, B.,Hu, Q.,Wu, J.,Bollen, M.,Shi, Y.
Structure and function of the two tandem WW domains of the pre-mRNA splicing factor FBP21 (formin-binding protein 21)
J.Biol.Chem., 284:25375-25387, 2009

PubMed Abstract: Human FBP21 (formin-binding protein 21) contains a matrin-type zinc finger and two tandem WW domains. It is a component of the spliceosomes and interacts with several established splicing factors. Here we demonstrate for the first time that FBP21 is an activator of pre-mRNA splicing in vivo and that its splicing activation function and interaction with the splicing factor SIPP1 (splicing factor that interacts with PQBP1 and PP1) are both mediated by the two tandem WW domains of group III. We determined the solution structure of the tandem WW domains of FBP21 and found that the WW domains recognize peptide ligands containing either group II (PPLP) or group III (PPR) motifs. The binding interfaces involve both the XP and XP2 grooves of the two WW domains. Significantly, the tandem WW domains of FBP21 are connected by a highly flexible region, enabling their simultaneous interaction with two proline-rich motifs of SIPP1. The strong interaction between SIPP1 and FBP21 can be explained by the conjugation of two low affinity interactions with the tandem WW domains. Our study provides a structural basis for understanding the molecular mechanism underlying the functional implication of FBP21 and the biological specificity of tandem WW domains.

PubMed: 19592703
DOI: 10.1074/jbc.M109.024828

実験手法

SOLUTION NMR