2JVB

Solution Structure of Catalytic Domain of yDcp2

2JVB の概要

• エントリーDOI: 10.2210/pdb2jvb/pdb
• 分子名称: mRNA-decapping enzyme subunit 2 (1 entity in total)
• 機能のキーワード: dcp2, mrna decay, decapping, cytoplasm, hydrolase, manganese, metal-binding, mrna processing, nonsense-mediated mrna decay, nucleus, phosphorylation, rna-binding
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasm, P-body: P53550
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17378.91

構造登録者

Deshmukh, M.,Gross, J. (登録日: 2007-09-16, 公開日: 2008-03-04, 最終更新日: 2024-05-29)

主引用文献

Deshmukh, M.V.,Jones, B.N.,Quang-Dang, D.U.,Flinders, J.,Floor, S.N.,Kim, C.,Jemielity, J.,Kalek, M.,Darzynkiewicz, E.,Gross, J.D.
mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2.
Mol.Cell, 29:324-336, 2008

PubMed Abstract: Cap hydrolysis by Dcp2 is a critical step in several eukaryotic mRNA decay pathways. Processing requires access to cap-proximal nucleotides and the coordinated assembly of a decapping mRNP, but the mechanism of substrate recognition and regulation by protein interactions have remained elusive. Using NMR spectroscopy and kinetic analyses, we show that yeast Dcp2 resolves interactions with the cap and RNA body using a bipartite surface that forms a channel intersecting the catalytic and regulatory Dcp1-binding domains. The interaction with cap is weak but specific and requires binding of the RNA body to a dynamic interface. The catalytic step is stimulated by Dcp1 and its interaction domain, likely through a substrate-induced conformational change. Thus, activation of the decapping mRNP is restricted by access to 5'-proximal nucleotides, a feature that could act as a checkpoint in mRNA metabolism.

PubMed: 18280238
DOI: 10.1016/j.molcel.2007.11.027

実験手法

SOLUTION NMR