2JUB

Solution structure of IPI*

2JUB の概要

• エントリーDOI: 10.2210/pdb2jub/pdb
• NMR情報: BMRB: 15438
• 分子名称: Internal protein I (1 entity in total)
• 機能のキーワード: ipi*, t4 phage, solution, endonuclease inhibitor
• 由来する生物種: Enterobacteria phage T4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8058.37

構造登録者

Rifat, D.,Wright, N.T.,Varney, K.M.,Weber, D.J.,Black, L.W. (登録日: 2007-08-17, 公開日: 2007-12-11, 最終更新日: 2024-05-08)

主引用文献

Rifat, D.,Wright, N.T.,Varney, K.M.,Weber, D.J.,Black, L.W.
Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target.
J.Mol.Biol., 375:720-734, 2008

PubMed Abstract: Phage T4 protects its DNA from the two-gene-encoded gmrS/gmrD (glucose-modified hydroxymethylcytosine restriction endonuclease) CT of pathogenic Escherichia coli, CT596, by injecting several hundred copies of the 76-amino-acid-residue nuclease inhibitor, IPI*, into the infected host. Here, the three-dimensional solution structure of mature IPI* is reported as determined by nuclear magnetic resonance techniques using 1290 experimental nuclear Overhauser effect and dipolar coupling constraints ( approximately 17 constraints per residue). Close examination of this oblate-shaped protein structure reveals a novel fold consisting of two small beta-sheets (beta1: B1 and B2; beta2: B3-B5) flanked at the N- and C-termini by alpha-helices (H1 and H2). Such a fold is very compact in shape and allows ejection of IPI* through the narrow 30-A portal and tail tube apertures of the virion without unfolding. Structural and dynamic measurements identify an exposed hydrophobic knob that is a putative gmrS/gmrD-binding site. A single gene from the uropathogenic E. coli UT189, which codes for a gmrS/gmrD-like UT fusion enzyme (with approximately 90% identity to the heterodimeric CT enzyme), has evolved IPI* inhibitor immunity. Analysis of the gmrS/gmrD restriction endonuclease enzyme family and its IPI* family phage antagonists reveals an evolutionary pathway that has elaborated a surprisingly diverse and specifically fitted set of coevolving attack and defense structures.

PubMed: 18037438
DOI: 10.1016/j.jmb.2007.10.064

実験手法

SOLUTION NMR