2JTX

NMR structure of the TFIIE-alpha carboxyl terminus

2JTX の概要

• エントリーDOI: 10.2210/pdb2jtx/pdb
• 分子名称: Transcription initiation factor IIE subunit alpha (1 entity in total)
• 機能のキーワード: tfiie, tfiih, activation domains, p53, transcription, transcription regulation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P29083
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11493.23

構造登録者

Di Lello, P.,Omichinski, J.G. (登録日: 2007-08-08, 公開日: 2007-12-11, 最終更新日: 2024-05-29)

主引用文献

Di Lello, P.,Miller Jenkins, L.M.,Mas, C.,Langlois, C.,Malitskaya, E.,Fradet-Turcotte, A.,Archambault, J.,Legault, P.,Omichinski, J.G.
p53 and TFIIEalpha share a common binding site on the Tfb1/p62 subunit of TFIIH.
Proc.Natl.Acad.Sci.Usa, 105:106-111, 2008

PubMed Abstract: The general transcription factor IIH is recruited to the transcription preinitiation complex through an interaction between its p62/Tfb1 subunit and the alpha-subunit of the general transcription factor IIE (TFIIEalpha). We have determined that the acidic carboxyl terminus of TFIIEalpha (TFIIEalpha(336-439)) directly binds the amino-terminal PH domain of p62/Tfb1 with nanomolar affinity. NMR mapping and mutagenesis studies demonstrate that the TFIIEalpha binding site on p62/Tfb1 is identical to the binding site for the second transactivation domain of p53 (p53 TAD2). In addition, we demonstrate that TFIIEalpha(336-439) is capable of competing with p53 for a common binding site on p62/Tfb1 and that TFIIEalpha(336-439) and the diphosphorylated form (pS46/pT55) of p53 TAD2 have similar binding constants. NMR structural studies reveal that TFIIEalpha(336-439) contains a small domain (residues 395-433) folded in a novel betabetaalphaalphaalpha topology. NMR mapping studies demonstrate that two unstructured regions (residues 377-393 and residues 433-439) located on either side of the folded domain appear to be required for TFIIEalpha(336-439) binding to p62/Tfb1 and that these two unstructured regions are held close to each other in three-dimensional space by the novel structured domain. We also demonstrate that, like p53, TFIIEalpha(336-439) can activate transcription in vivo. These results point to an important interplay between the general transcription factor TFIIEalpha and the tumor suppressor protein p53 in regulating transcriptional activation that may be modulated by the phosphorylation status of p53.

PubMed: 18160537
DOI: 10.1073/pnas.0707892105

実験手法

SOLUTION NMR