2JTM

Solution structure of Sso6901 from Sulfolobus solfataricus P2

2JTM の概要

• エントリーDOI: 10.2210/pdb2jtm/pdb
• NMR情報: BMRB: 15415
• 分子名称: Putative uncharacterized protein (1 entity in total)
• 機能のキーワード: sh3-like, dna binding protein
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6677.91

構造登録者

Feng, Y.,Guo, L.,Huang, L.,Wang, J. (登録日: 2007-08-03, 公開日: 2008-04-29, 最終更新日: 2024-05-15)

主引用文献

Guo, L.,Feng, Y.,Zhang, Z.,Yao, H.,Luo, Y.,Wang, J.,Huang, L.
Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea
Nucleic Acids Res., 36:1129-1137, 2008

PubMed Abstract: Archaea contain a variety of chromatin proteins consistent with the evolution of different genome packaging mechanisms. Among the two main kingdoms in the Archaea, Euryarchaeota synthesize histone homologs, whereas Crenarchaeota have not been shown to possess a chromatin protein conserved at the kingdom level. We report the identification of Cren7, a novel family of chromatin proteins highly conserved in the Crenarchaeota. A small, basic, methylated and abundant protein, Cren7 displays a higher affinity for double-stranded DNA than for single-stranded DNA, constrains negative DNA supercoils and is associated with genomic DNA in vivo. The solution structure and DNA-binding surface of Cren7 from the hyperthermophilic crenarchaeon Sulfolobus solfataricus were determined by NMR. The protein adopts an SH3-like fold. It interacts with duplex DNA through a beta-sheet and a long flexible loop, presumably resulting in DNA distortions through intercalation of conserved hydrophobic residues into the DNA structure. These data suggest that the crenarchaeal kingdom in the Archaea shares a common strategy in chromatin organization.

PubMed: 18096617
DOI: 10.1093/nar/gkm1128

実験手法

SOLUTION NMR