2JTA

NMR structure of immunosuppressory ubiquitin fragment is similar to related ubiquitin region.

2JTA の概要

• エントリーDOI: 10.2210/pdb2jta/pdb
• 分子名称: 10-mer ubiquitin peptide (1 entity in total)
• 機能のキーワード: alpha helix, immunosuppression, ubiquitin, signaling protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1169.22

構造登録者

Jaremko, M.,Jaremko, L.,Zhukov, I.,Cebrat, M. (登録日: 2007-07-21, 公開日: 2008-07-29, 最終更新日: 2024-05-29)

主引用文献

Jaremko, L.,Jaremko, M.,Pasikowski, P.,Cebrat, M.,Stefanowicz, P.,Lisowski, M.,Artym, J.,Zimecki, M.,Zhukov, I.,Szewczuk, Z.
The immunosuppressive activity and solution structures of ubiquitin fragments.
Biopolymers, 91:423-431, 2009

PubMed Abstract: Recently, ubiquitin was suggested as a promising anti-inflammatory protein therapeutic. We found that a peptide fragment corresponding to the ubiquitin(50-59) sequence (LEDGRTLSDY) possessed the immunosuppressive activity comparable with that of ubiquitin. CD and NMR spectroscopies were used to determine the conformational preferences of LEDGRTLSDY in solution. The peptide mixture, obtained by pepsin digestion of ubiquitin, was even more potent than the intact protein. Although the peptide exhibited a well-defined conformation in methanol, its structure was distinct from the corresponding 50-59 fragment in the native ubiquitin molecule. (c) 2009 Wiley Periodicals, Inc. Biopolymers 91: 423-431, 2009.

PubMed: 19213045
DOI: 10.1002/bip.21160

実験手法

SOLUTION NMR