2JSO

Antimicrobial resistance protein

Summary for 2JSO

• Entry DOI: 10.2210/pdb2jso/pdb
• NMR Information: BMRB: 15371
• Descriptor: Polymyxin resistance protein pmrD (1 entity in total)
• Functional Keywords: protein, antibiotic resistance, transcription, signaling protein
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 9885.54

Authors

Jin, C.,Fu, W. (deposition date: 2007-07-10, release date: 2007-09-04, Last modification date: 2024-05-01)

Primary citation

Fu, W.,Yang, F.,Kang, X.,Zhang, X.,Li, Y.,Xia, B.,Jin, C.
First structure of the polymyxin resistance proteins.
Biochem.Biophys.Res.Commun., 361:1033-1037, 2007

PubMed Abstract: PmrA/PmrB and PhoP/PhoQ are a pair of two-component systems (TCSs) that allow the Gram-negative bacteria to survive the cationic antimicrobial peptide polymyxin B. The two TCSs are linked by the polymyxin resistance protein, PmrD. The PhoP-activated PmrD protects the phosphorylated response regulator PmrA from dephosphorylation, and promotes the transcription of PmrA-activated genes responsible for polymyxin resistance. PmrD is the first protein identified to mediate the connectivity between two TCSs by protecting the phosphorylated response regulator of the downstream TCS. PmrD shows no homology to proteins with known structures. We present here the solution structure of PmrD from Escherichia coli, the first three-dimensional structure of the PmrD family. Our study provides the structural basis of the novel interacting mechanism of bacterial two-component signal-transduction systems.

PubMed: 17686460
DOI: 10.1016/j.bbrc.2007.07.144

Experimental method

SOLUTION NMR