2JSI

11-23 obestatin fragment in DPC/SDS micellar solution

2JSI の概要

• エントリーDOI: 10.2210/pdb2jsi/pdb
• 関連するPDBエントリー: 2JSH 2JSJ
• 分子名称: Appetite-regulating hormone, Obestatin (1 entity in total)
• 機能のキーワード: 11-23 obestatin fragment, micellar solution, dpc, sds, alternative splicing, amidation, hormone, lipoprotein, secreted
• 細胞内の位置: Secreted: Q9EQX0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1428.58

構造登録者

D'Ursi, A.M.,Scrima, M.,Esposito, C.,Campiglia, P. (登録日: 2007-07-05, 公開日: 2008-10-21, 最終更新日: 2024-11-06)

主引用文献

Scrima, M.,Campiglia, P.,Esposito, C.,Gomez-Monterrey, I.,Novellino, E.,D'Ursi, A.M.
Obestatin conformational features: a strategy to unveil obestatin's biological role?
Biochem.Biophys.Res.Commun., 363:500-505, 2007

PubMed Abstract: Obestatin and its derivative Ob(11-23) are recently discovered peptides produced in the rat stomach. They have proven to be involved in the regulation of energy balance, inhibiting feeding, causing reductions in food intake, body weight and jejunal contraction in rodents. The G-protein coupled receptor, GPR39, was originally proposed as being an obestatin target receptor, but this remains controversial. As such, the molecular mechanism for obestatin's effects in vivo is still uncertain. Here we report the CD and NMR conformational analysis of obestatin and Ob(11-23). Both peptides assume a regular secondary structure in the C-terminal region of the molecule. In this region, structural elements similar to other GPCR binding neuropeptides support the identity of obestatin as a new and functionally autonomous GPCR ligand. Conversely sequence and conformational specificity point to a new farmacoforic structure, on which innovative derivatives with a potential role in the treatment of obesity can be designed and synthetized.

PubMed: 17904104
DOI: 10.1016/j.bbrc.2007.08.200

実験手法

SOLUTION NMR