2JSA

Solution structure of Caenopore-5 (81 Pro Trans confomer)

2JSA の概要

• エントリーDOI: 10.2210/pdb2jsa/pdb
• 関連するPDBエントリー: 2JS9
• NMR情報: BMRB: 15358
• 分子名称: Saposin-like protein family protein 5 (1 entity in total)
• 機能のキーワード: caenopore-5, saposin-like fold, antimicrobial protein
• 由来する生物種: Caenorhabditis elegans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11010.46

構造登録者

Mysliwy, J.,Grotzinger, J. (登録日: 2007-07-02, 公開日: 2008-10-14, 最終更新日: 2024-10-16)

主引用文献

Mysliwy, J.,Dingley, A.J.,Stanisak, M.,Jung, S.,Lorenzen, I.,Roeder, T.,Leippe, M.,Grotzinger, J.
Caenopore-5: the three-dimensional structure of an antimicrobial protein from Caenorhabditis elegans.
Dev.Comp.Immunol., 34:323-330, 2010

PubMed Abstract: The caenopore-5 protein encoded by the spp-5 gene is one of the 33 caenopores identified in Caenorhabditis elegans and is a pore-forming peptide which plays an important role in the elimination of Escherichia coli ingested by the worm. Thus, caenopore-5 appears to contribute to the nutrition of the worm while simultaneously protecting the organism against pathogens. Here, three-dimensional heteronuclear NMR spectroscopy was used to solve the solution structure of caenopore-5. The NMR data revealed that two conformers of caenopore-5 exist in solution which differ by the isomerization of the peptide bond of Pro-81. The overall structure of the two caenopore-5 conformers consists of five amphiphatic helices connected by three disulfide bonds. The five helices are arranged in a folded leaf which is the characteristic signature of the SAPLIP family. The structure presented here is the first of an effector protein of the defensive system elucidated for the well-known model organism C. elegans.

PubMed: 19917307
DOI: 10.1016/j.dci.2009.11.003

実験手法

SOLUTION NMR