2JRG

NMR solution structure of the anticodon of E. coli TRNA-VAL3 with 2 modifications (cmo5U34 M6A37)

2JRG の概要

• エントリーDOI: 10.2210/pdb2jrg/pdb
• 関連するPDBエントリー: 2JR4 2JRQ
• NMR情報: BMRB: 15331
• 分子名称: 5'-R(*CP*CP*UP*CP*CP*CP*UP*(CM0)P*AP*CP*(6MZ)P*AP*GP*GP*AP*GP*G)-3' (1 entity in total)
• 機能のキーワード: e.coli, valine, trna, anticodon stem loop, trna domain, rna hairpin, uridine 5-oxyacetic acid, cmo5u, n6-methyladenosine, m6a, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5490.34

構造登録者

Vendeix, F.A.,Dziergowska, A.,Gustilo, E.M.,Graham, W.D.,Sproat, B.,Malkiewicz, A.,Agris, P.F. (登録日: 2007-06-26, 公開日: 2007-07-24, 最終更新日: 2023-12-20)

主引用文献

Vendeix, F.A.,Dziergowska, A.,Gustilo, E.M.,Graham, W.D.,Sproat, B.,Malkiewicz, A.,Agris, P.F.
Anticodon domain modifications contribute order to tRNA for ribosome-mediated codon binding.
Biochemistry, 47:6117-6129, 2008

PubMed Abstract: The accuracy and efficiency with which tRNA decodes genomic information into proteins require posttranscriptional modifications in or adjacent to the anticodon. The modification uridine-5-oxyacetic acid (cmo (5)U 34) is found at wobble position 34 in a single isoaccepting tRNA species for six amino acids, alanine, leucine, proline, serine, threonine, and valine, each having 4-fold degenerate codons. cmo (5)U 34 makes possible the decoding of 24 codons by just six tRNAs. The contributions of this important modification to the structures and codon binding affinities of the unmodified and fully modified anticodon stem and loop domains of tRNA (Val3) UAC (ASL (Val3) UAC) were elucidated. The stems of the unmodified ASL (Val3) UAC and that with cmo (5)U 34 and N (6)-methyladenosine, m (6)A 37, adopted an A-form RNA conformation (rmsd approximately 0.6 A) as determined with NMR spectroscopy and torsion-angle molecular dynamics. However, the UV hyperchromicity, circular dichroism ellipticity, and structural analyses indicated that the anticodon modifications enhanced order in the loop. ASL (Val3) UAC-cmo (5)U 34;m (6)A 37 exhibited high affinities for its cognate and wobble codons GUA and GUG, and for GUU in the A-site of the programmed 30S ribosomal subunit, whereas the unmodified ASL (Val3) UAC bound less strongly to GUA and not at all to GUG and GUU. Together with recent crystal structures of ASL (Val3) UAC-cmo (5)U 34;m (6)A 37 bound to all four of the valine codons in the A-site of the ribosome's 30S subunit, these results clearly demonstrate that the xo (5)U 34-type modifications order the anticodon loop prior to A-site codon binding for an expanded codon reading, possibly reducing an entropic energy barrier to codon binding.

PubMed: 18473483
DOI: 10.1021/bi702356j

実験手法

SOLUTION NMR