2JRB

C-terminal domain of ORF1p from mouse LINE-1

2JRB の概要

• エントリーDOI: 10.2210/pdb2jrb/pdb
• NMR情報: BMRB: 15325
• 分子名称: ORF 1 protein (1 entity in total)
• 機能のキーワード: rna binding protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10430.11

構造登録者

Januszyk, K.,Clubb, R. (登録日: 2007-06-21, 公開日: 2007-07-17, 最終更新日: 2023-12-20)

主引用文献

Januszyk, K.,Li, P.W.,Villareal, V.,Branciforte, D.,Wu, H.,Xie, Y.,Feigon, J.,Loo, J.A.,Martin, S.L.,Clubb, R.T.
Identification and solution structure of a highly conserved C-terminal domain within ORF1p required for retrotransposition of long interspersed nuclear element-1.
J.Biol.Chem., 282:24893-24904, 2007

PubMed Abstract: Long interspersed nuclear element-1 (LINE-1 or L1) retrotransposons comprise a large fraction of the human and mouse genomes. The mobility of these successful elements requires the protein encoded by open reading frame-1 (ORF1p), which binds single-stranded RNA with high affinity and functions as a nucleic acid chaperone. In this report, we have used limited proteolysis, filter binding, and NMR spectroscopy to characterize the global structure of ORF1p and the three-dimensional structure of a highly conserved RNA binding domain. ORF1p contains three structured regions, a coiled-coil domain, a middle domain of unknown function, and a C-terminal domain (CTD). We show that high affinity RNA binding by ORF1p requires the CTD and residues within an amino acid protease-sensitive segment that joins the CTD to the middle domain. Insights in the mechanism of RNA binding were obtained by determining the solution structure of the CTD, which is shown to adopt a novel fold consisting of a three-stranded beta sheet that is packed against three alpha-helices. An RNA binding surface on the CTD has been localized using chemical shift perturbation experiments and is proximal to residues previously shown to be essential for retrotransposition, RNA binding, and chaperone activity. A similar structure and mechanism of RNA binding is expected for all vertebrate long interspersed nuclear element-1 elements, since residues encoding the middle, protease-sensitive segment, and CTD are highly conserved.

PubMed: 17569664
DOI: 10.1074/jbc.M702023200

実験手法

SOLUTION NMR