2JQY

Outer Membrane Protein G

2JQY の概要

• エントリーDOI: 10.2210/pdb2jqy/pdb
• 分子名称: Outer membrane protein G (1 entity in total)
• 機能のキーワード: ompg, membrane protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell outer membrane; Multi-pass membrane protein: P76045
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32805.33

構造登録者

Liang, B.,Tamm, L.K. (登録日: 2007-06-15, 公開日: 2007-10-09, 最終更新日: 2023-12-20)

主引用文献

Liang, B.,Tamm, L.K.
Structure of outer membrane protein G by solution NMR spectroscopy.
Proc.Natl.Acad.Sci.USA, 104:16140-16145, 2007

PubMed Abstract: The bacterial outer membrane protein G (OmpG), a monomeric pH-gated porin, was overexpressed in Escherichia coli and refolded in beta-octyl glucoside micelles. After transfer into dodecylphosphocholine micelles, the solution structure of OmpG was determined by solution NMR spectroscopy at pH 6.3. Complete backbone assignments were obtained for 234 of 280 residues based on CA, CB, and CO connection pathways determined from a series of TROSY-based 3D experiments at 800 MHz. The global fold of the 14-stranded beta-barrel was determined based on 133 long-range NOEs observed between neighboring strands and local chemical shift and NOE information. The structure of the barrel is very similar to previous crystal structures, but the loops of the solution structure are quite flexible.

PubMed: 17911261
DOI: 10.1073/pnas.0705466104

実験手法

SOLUTION NMR