2JQR

Solution model of crosslinked complex of cytochrome c and adrenodoxin

2JQR の概要

• エントリーDOI: 10.2210/pdb2jqr/pdb
• 関連するPDBエントリー: 1AYF 1YCC
• NMR情報: BMRB: 7390,15301
• 分子名称: Cytochrome c iso-1, Adrenodoxin, mitochondrial, HEME C, ... (4 entities in total)
• 機能のキーワード: electron transport, cytochrome c, adrenodoxin, crosslinked complex, 2fe2s ferredoxin, pseudocontact shift, paramagnetic relaxation enhancement, encounter complex
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Mitochondrion intermembrane space: P00044; Mitochondrion matrix: P00257
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24505.26

構造登録者

Xu, X.,Reinle, W.,Hannemann, F.,Konarev, P.V.,Svergun, D.I.,Bernhardt, R.,Ubbink, M. (登録日: 2007-06-07, 公開日: 2008-04-22, 最終更新日: 2024-10-30)

主引用文献

Xu, X.,Reinle, W.,Hannemann, F.,Konarev, P.V.,Svergun, D.I.,Bernhardt, R.,Ubbink, M.
Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy
J.Am.Chem.Soc., 130:6395-6403, 2008

PubMed Abstract: In the general view of protein-complex formation, a transient and dynamic encounter complex proceeds to form a more stable, well-defined, and active form. In weak protein complexes, however, the encounter state can represent a significant population of the complex. The redox proteins adrenodoxin (Adx) and cytochrome c (C c) associate to form such a weak and short-lived complex, which is nevertheless active in electron transfer. To study the conformational freedom within the protein complex, the native complex has been compared to a cross-linked counterpart by using solution scattering and NMR spectroscopy. Oligomerization behavior of the native complex in solution revealed by small-angle X-ray scattering indicates a stochastic nature of complex formation. For the cross-linked complex, interprotein paramagnetic effects are observed, whereas for the native complex, extensive averaging occurs, consistent with multiple orientations of the proteins within the complex. Simulations show that C c samples about half of the surface area of adrenodoxin. It is concluded that the complex of Adx/C c is entirely dynamic and can be considered as a pure encounter complex.

PubMed: 18439013
DOI: 10.1021/ja7101357

実験手法

SOLUTION NMR