2JQD

Structure of the Leucine-Rich Repeat domain of LANP

2JQD の概要

• エントリーDOI: 10.2210/pdb2jqd/pdb
• 分子名称: Acidic leucine-rich nuclear phosphoprotein 32 family member A (1 entity in total)
• 機能のキーワード: lanp/anp32a, lrr domain, phosphoprotein, pp2a inhibitor, tumor suppression, transcriptional regulation, rna shuttling, apoptosis, cerebellar morphogenesis, gene regulation, protein binding
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: O35381
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19213.93

構造登録者

de Chiara, C.,Pastore, A. (登録日: 2007-05-31, 公開日: 2008-04-22, 最終更新日: 2024-05-29)

主引用文献

de Chiara, C.,Menon, R.P.,Pastore, A.
Structural bases for recognition of Anp32/LANP proteins
Febs J., 275:2548-2560, 2008

PubMed Abstract: The leucine-rich repeat acidic nuclear protein (Anp32a/LANP) belongs to a family of evolutionarily-conserved phosphoproteins involved in a complex network of protein-protein interactions. In an effort to understand the cellular role, we have investigated the mode of interaction of Anp32a with its partners. As a prerequisite, we solved the structure in solution of the evolutionarily conserved N-terminal leucine-rich repeat (LRR) domain and modeled its interactions with other proteins, taking PP2A as a paradigmatic example. The interaction between the Anp32a LRR domain and the AXH domain of ataxin-1 was probed experimentally. The two isolated and unmodified domains bind with very weak (millimolar) affinity, thus suggesting the necessity either for an additional partner (e.g. other regions of either or both proteins or a third molecule) or for a post-translational modification. Finally, we identified by two-hybrid screening a new partner of the LRR domain, i.e. the microtubule plus-end tracking protein Clip 170/Restin, known to regulate the dynamic properties of microtubules and to be associated with severe human pathologies.

PubMed: 18410380
DOI: 10.1111/j.1742-4658.2008.06403.x

実験手法

SOLUTION NMR