2JQC

A L-amino acid mutant of a D-amino acid containing conopeptide

2JQC の概要

• エントリーDOI: 10.2210/pdb2jqc/pdb
• 関連するPDBエントリー: 2EFZ 2JQB 2YYF
• NMR情報: BMRB: 15273
• 分子名称: L-mr12 (1 entity in total)
• 機能のキーワード: m cono-toxin, mr12, nmr solution structure, toxin
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1934.05

構造登録者

Huang, F.,Du, W.,Han, Y.,Wang, C.,Chi, C. (登録日: 2007-05-31, 公開日: 2008-04-15, 最終更新日: 2023-12-20)

主引用文献

Han, Y.,Huang, F.,Jiang, H.,Liu, L.,Wang, Q.,Wang, Y.,Shao, X.,Chi, C.,Du, W.,Wang, C.
Purification and structural characterization of a d-amino acid-containing conopeptide, conomarphin, from Conus marmoreus
Febs J., 275:1976-1987, 2008

PubMed Abstract: Cone snails, a group of gastropod animals that inhabit tropical seas, are capable of producing a mixture of peptide neurotoxins, namely conotoxins, for defense and predation. Conotoxins are mainly disulfide-rich short peptides that act on different ion channels, neurotransmitter receptors, or transporters in the nervous system. They exhibit highly diverse compositions, structures, and biological functions. In this work, a novel Cys-free 15-residue conopeptide from Conus marmoreus was purified and designated as conomarphin. Conomarphin is unique because of its D-configuration Phe at the third residue from the C-terminus, which was identified using HPLC by comparing native conomarphin fragments and the corresponding synthetic peptides cleaved by different proteases. Surprisingly, the cDNA-encoded precursor of conomarphin was found to share the conserved signal peptide with other M-superfamily conotoxins, clearly indicating that conomarphin should belong to the M-superfamily, although conomarphin shares no homology with other six-Cys-containing M-superfamily conotoxins. Furthermore, NMR spectroscopy experiments established that conomarphin adopts a well-defined structure in solution, with a tight loop in the middle of the peptide and a short 3(10)-helix at the C-terminus. By contrast, no loop in L-Phe13-conomarphin was found, which suggests that D-Phe13 is essential for the structure of conomarphin. In conclusion, conomarphin may represent a new conotoxin family, whose biological activity remains to be identified.

PubMed: 18355315
DOI: 10.1111/j.1742-4658.2008.06352.x

実験手法

SOLUTION NMR