2JPP

Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA

2JPP の概要

• エントリーDOI: 10.2210/pdb2jpp/pdb
• NMR情報: BMRB: 15257
• 分子名称: RNA (5'-R(*GP*GP*GP*CP*UP*UP*CP*AP*CP*GP*GP*AP*UP*GP*AP*AP*GP*CP*CP*C)-3'), Translational repressor (2 entities in total)
• 機能のキーワード: rna recognition, protein/rna, csra, rsma, shine-dalgarno, translation-rna complex, translation/rna
• 由来する生物種: Pseudomonas fluorescens
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 28571.69

構造登録者

Schubert, M.,Lapouge, K.,Duss, O.,Oberstrass, F.C.,Jelesarov, I.,Haas, D.,Allain, F.H.-T. (登録日: 2007-05-21, 公開日: 2007-08-21, 最終更新日: 2023-12-20)

主引用文献

Schubert, M.,Lapouge, K.,Duss, O.,Oberstrass, F.C.,Jelesarov, I.,Haas, D.,Allain, F.H.-T.
Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA/CsrA
Nat.Struct.Mol.Biol., 14:807-813, 2007

PubMed Abstract: Proteins of the RsmA/CsrA family are global translational regulators in many bacterial species. We have determined the solution structure of a complex formed between the RsmE protein, a member of this family from Pseudomonas fluorescens, and a target RNA encompassing the ribosome-binding site of the hcnA gene. The RsmE homodimer with its two RNA-binding sites makes optimal contact with an 5'-A/UCANGGANGU/A-3' sequence in the mRNA. When tightly gripped by RsmE, the ANGGAN core folds into a loop, favoring the formation of a 3-base-pair stem by flanking nucleotides. We validated these findings by in vivo and in vitro mutational analyses. The structure of the complex explains well how, by sequestering the Shine-Dalgarno sequence, the RsmA/CsrA proteins repress translation.

PubMed: 17704818
DOI: 10.1038/nsmb1285

実験手法

SOLUTION NMR