2JPH
NMR solution structure of the Rho GTPase binding domain of human plexin-b1
Summary for 2JPH
Entry DOI | 10.2210/pdb2jph/pdb |
Descriptor | Plexin-B1 (1 entity in total) |
Functional Keywords | protein, ubiquitin fold, signaling protein, protein binding |
Biological source | Homo sapiens (human) |
Cellular location | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted: O43157 |
Total number of polymer chains | 1 |
Total formula weight | 13590.61 |
Authors | |
Primary citation | Tong, Y.,Hota, P.K.,Hamaneh, M.B.,Buck, M. Insights into Oncogenic Mutations of Plexin-B1 Based on the Solution Structure of the Rho GTPase Binding Domain Structure, 16:246-258, 2008 Cited by PubMed Abstract: The plexin family of transmembrane receptors are important for axon guidance, angiogenesis, but also in cancer. Recently, plexin-B1 somatic missense mutations were found in both primary tumors and metastases of breast and prostate cancers, with several mutations mapping to the Rho GTPase binding domain (RBD) in the cytoplasmic region of the receptor. Here we present the NMR solution structure of this domain, confirming that the protein has both a ubiquitin-like fold and surface features. Oncogenic mutations T1795A and T1802A are located in a loop region, perturb the average structure locally, and have no effect on Rho GTPase binding affinity. Mutations L1815F and L1815P are located at the Rho GTPase binding site and are associated with a complete loss of binding for Rac1 and Rnd1. Both are found to disturb the conformation of the beta3-beta4 sheet and the orientation of surrounding side chains. Our study suggests that the oncogenic behavior of the mutants can be rationalized with reference to the structure of the RBD of plexin-B1. PubMed: 18275816DOI: 10.1016/j.str.2007.12.012 PDB entries with the same primary citation |
Experimental method | SOLUTION NMR |
Structure validation
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