2JP8

Angiotensin 1-7

2JP8 の概要

• エントリーDOI: 10.2210/pdb2jp8/pdb
• 分子名称: Angiotensin-(1-7) (1 entity in total)
• 機能のキーワード: bend, signaling protein
• 細胞内の位置: Secreted: P01019
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 901.02

構造登録者

Lula, I.,Denadai, A.L.,Resende, J.M.,de Souza, F.B.,de Lima, G.F.,Pilo-Veloso, D.,Heine, T.,Duarte, H.A.,Santos, R.A.S.,Sinesterra, R.D. (登録日: 2007-04-27, 公開日: 2007-10-30, 最終更新日: 2023-12-20)

主引用文献

Lula, I.,Denadai, A.L.,Resende, J.M.,de Sousa, F.B.,de Lima, G.F.,Pilo-Veloso, D.,Heine, T.,Duarte, H.A.,Santos, R.A.,Sinisterra, R.D.
Study of angiotensin-(1-7) vasoactive peptide and its beta-cyclodextrin inclusion complexes: complete sequence-specific NMR assignments and structural studies
Peptides, 28:2199-2210, 2007

PubMed Abstract: We report the complete sequence-specific hydrogen NMR assignments of vasoactive peptide angiotensin-(1-7) (Ang-(1-7)). Assignments of the majority of the resonances were accomplished by COSY, TOCSY, and ROESY peak coordinates at 400MHz and 600MHz. Long-side-chain amino acid spin system identification was facilitated by long-range coherence transfer experiments (TOCSY). Problems with overlapped resonance signals were solved by analysis of heteronuclear 2D experiments (HSQC and HMBC). Nuclear Overhauser effects (NOE) results were used to probe peptide conformation. We show that the inclusion of the angiotensin-(1-7) tyrosine residue is favored in inclusion complexes with beta-cyclodextrin. QM/MM simulations at the DFTB/UFF level confirm the experimental NMR findings and provide detailed structural information on these compounds in aqueous solution.

PubMed: 17904691
DOI: 10.1016/j.peptides.2007.08.011

実験手法

SOLUTION NMR