2JOU

NMR structure of Mini-B, an N-terminal- C-terminal construct from human Surfactant Protein-B (SP-B), in Hexafluoroisopropanol (HFIP)

「2A2H」から置き換えられました

2JOU の概要

• エントリーDOI: 10.2210/pdb2jou/pdb
• 関連するPDBエントリー: 1RG3 1RG4
• NMR情報: BMRB: 6741
• 分子名称: Pulmonary surfactant-associated protein B (1 entity in total)
• 機能のキーワード: mini-b, sp-b, surfactant protein b, lipid associated protein, surface active protein
• 細胞内の位置: Secreted, extracellular space, surface film: P07988
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3934.02

構造登録者

Booth, V.,Sarker, M.,Keough, K.M.W.,Waring, A.J.,Walther, F.J. (登録日: 2007-03-26, 公開日: 2007-04-10, 最終更新日: 2023-12-20)

主引用文献

Sarker, M.,Waring, A.J.,Walther, F.J.,Keough, K.M.,Booth, V.
Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles
Biochemistry, 46:11047-11056, 2007

PubMed Abstract: Surfactant protein B (SP-B) is essential for normal lung surfactant function, which is in itself essential to life. However, the molecular basis for SP-B's activity is not understood and a high-resolution structure for SP-B has not been determined. Mini-B is a 34-residue peptide with internal disulfide linkages that is composed of the N- and C-terminal helical regions of SP-B. It has been shown to retain similar activity to full-length SP-B in certain in vitro and in vivo studies. We have used solution NMR to determine the structure of Mini-B in the presence of micelles composed of the anionic detergent sodium dodecyl sulfate (SDS). Under these conditions, Mini-B forms two alpha-helices connected by an unstructured loop. Mini-B possesses a strikingly amphipathic surface with a large positively charged patch on one face of the peptide and a large hydrophobic patch on the opposite face. A tryptophan side chain extends outward from the peptide in a position to interact with lipids at the polar/apolar interface. Interhelix interactions are stabilized by both disulfide bonds and by interleaving of hydrophobic side chains from the two helices.

PubMed: 17845058
DOI: 10.1021/bi7011756

実験手法

SOLUTION NMR