2JON

Solution structure of the C-terminal domain Ole e 9

Summary for 2JON

• Entry DOI: 10.2210/pdb2jon/pdb
• Descriptor: Beta-1,3-glucanase (1 entity in total)
• Functional Keywords: olive pollen, allergen
• Biological source: Olea europaea (common olive)
• Total number of polymer chains: 1
• Total formula weight: 10595.59

Authors

Trevino, M.A.,Palomares, O.,Castrillo, I.,Villalba, M.,Rodriguez, R.,Rico, M.,Santoro, J.,Bruix, M. (deposition date: 2007-03-14, release date: 2008-01-29, Last modification date: 2023-12-20)

Primary citation

Trevino, M.A.,Palomares, O.,Castrillo, I.,Villalba, M.,Rodriguez, R.,Rico, M.,Santoro, J.,Bruix, M.
Solution structure of the C-terminal domain of Ole e 9, a major allergen of olive pollen
Protein Sci., 17:371-376, 2008

PubMed Abstract: Ole e 9 is an olive pollen allergen belonging to group 2 of pathogenesis-related proteins. The protein is composed of two immunological independent domains: an N-terminal domain (NtD) with 1,3-beta-glucanase activity, and a C-terminal domain (CtD) that binds 1,3-beta-glucans. We have determined the three-dimensional structure of CtD-Ole e 9 (101 amino acids), which consists of two parallel alpha-helices forming an angle of approximately 55 degrees , a small antiparallel beta-sheet with two short strands, and a 3-10 helix turn, all connected by long coil segments, resembling a novel type of folding among allergens. Two regions surrounded by aromatic residues (F49, Y60, F96, Y91 and Y31, H68, Y65, F78) have been localized on the protein surface, and a role for sugar binding is suggested. The epitope mapping of CtD-Ole e 9 shows that B-cell epitopes are mainly located on loops, although some of them are contained in secondary structural elements. Interestingly, the IgG and IgE epitopes are contiguous or overlapped, rather than coincident. The three-dimensional structure of CtD-Ole e 9 might help to understand the underlying mechanism of its biochemical function and to determine possible structure-allergenicity relationships.

PubMed: 18096638
DOI: 10.1110/ps.073230008

Experimental method

SOLUTION NMR