2JNF

Solution structure of fly troponin C, isoform F1

2JNF の概要

• エントリーDOI: 10.2210/pdb2jnf/pdb
• NMR情報: BMRB: 6081
• 分子名称: Troponin C (1 entity in total)
• 機能のキーワード: stretch activated muscle contraction, troponin c, ef-hand, lethocerus indicus, metal binding protein
• 由来する生物種: Lethocerus indicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17589.30

構造登録者

De Nicola, G.F.,Bullard, B.,Pastore, A. (登録日: 2007-01-18, 公開日: 2007-08-07, 最終更新日: 2023-12-20)

主引用文献

De Nicola, G.,Burkart, C.,Qiu, F.,Agianian, B.,Labeit, S.,Martin, S.,Bullard, B.,Pastore, A.
The structure of lethocerus troponin C: insights into the mechanism of stretch activation in muscles
Structure, 15:813-824, 2007

PubMed Abstract: To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.

PubMed: 17637342
DOI: 10.1016/j.str.2007.05.007

実験手法

SOLUTION NMR