2JMZ

Solution structure of a KlbA intein precursor from Methanococcus jannaschii

2JMZ の概要

• エントリーDOI: 10.2210/pdb2jmz/pdb
• 分子名称: Hypothetical protein MJ0781 (1 entity in total)
• 機能のキーワード: protein, unknown function
• 由来する生物種: Methanocaldococcus jannaschii
• 細胞内の位置: Membrane; Single-pass membrane protein (Potential): Q58191
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21441.24

構造登録者

Johnson, M.A.,Southworth, M.W.,Herrmann, T.,Brace, L.,Perler, F.B.,Wuthrich, K.A. (登録日: 2006-12-14, 公開日: 2007-07-10, 最終更新日: 2023-12-20)

主引用文献

Johnson, M.A.,Southworth, M.W.,Herrmann, T.,Brace, L.,Perler, F.B.,Wuthrich, K.
NMR structure of a KlbA intein precursor from Methanococcus jannaschii
Protein Sci., 16:1316-1328, 2007

PubMed Abstract: Certain proteins of unicellular organisms are translated as precursor polypeptides containing inteins (intervening proteins), which are domains capable of performing protein splicing. These domains, in conjunction with a single residue following the intein, catalyze their own excision from the surrounding protein (extein) in a multistep reaction involving the cleavage of two intein-extein peptide bonds and the formation of a new peptide bond that ligates the two exteins to yield the mature protein. We report here the solution NMR structure of a 186-residue precursor of the KlbA intein from Methanococcus jannaschii, comprising the intein together with N- and C-extein segments of 7 and 11 residues, respectively. The intein is shown to adopt a single, well-defined globular domain, representing a HINT (Hedgehog/Intein)-type topology. Fourteen beta-strands are arranged in a complex fold that includes four beta-hairpins and an antiparallel beta-ribbon, and there is one alpha-helix, which is packed against the beta-ribbon, and one turn of 3(10)-helix in the loop between the beta-strands 8 and 9. The two extein segments show increased disorder, and form only minimal nonbonding contacts with the intein domain. Structure-based mutation experiments resulted in a proposal for functional roles of individual residues in the intein catalytic mechanism.

PubMed: 17586768
DOI: 10.1110/ps.072816707

実験手法

SOLUTION NMR