2JML

Solution structure of the N-terminal domain of CarA repressor

2JML の概要

• エントリーDOI: 10.2210/pdb2jml/pdb
• NMR情報: BMRB: 15023
• 分子名称: DNA BINDING DOMAIN/TRANSCRIPTIONAL REGULATOR (1 entity in total)
• 機能のキーワード: anti-repressor, merr, carotenogenesis, transcription
• 由来する生物種: Myxococcus xanthus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9471.79

構造登録者

Jimenez, M.,Padmanabhan, S.,Gonzalez, C.,Perez-Marin, M.C.,Elias-Arnanz, M.,Murillo, F.J.,Rico, M. (登録日: 2006-11-20, 公開日: 2007-02-13, 最終更新日: 2023-12-20)

主引用文献

Navarro-Aviles, G.,Jimenez, M.A.,Perez-Marin, M.C.,Gonzalez, C.,Rico, M.,Murillo, F.J.,Elias-Arnanz, M.,Padmanabhan, S.
Structural basis for operator and antirepressor recognition by Myxococcus xanthus CarA repressor.
Mol.Microbiol., 63:980-994, 2007

PubMed Abstract: Blue light induces carotenogenesis in Myxococcus xanthus. The carB operon encodes all but one of the structural genes involved, and its expression is regulated by the CarA-CarS repressor-antirepressor pair. In the dark, CarA-operator binding represses carB. CarS, produced on illumination, interacts physically with CarA to dismantle the CarA-operator complex and activate carB. Both operator and CarS bind to the autonomously folded N-terminal domain of CarA, CarA(Nter), which in excess represses carB. Here, we report the NMR structure of CarA(Nter), and map residues that interact with operator and CarS by NMR chemical shift perturbations, and in vivo and in vitro analyses of site-directed mutants. We show CarA(Nter) adopts the winged-helix topology of MerR-family DNA-binding domains, and conserves the majority of the helix-turn-helix and wing contacts with DNA. Tellingly, helix alpha2 in CarA, a key element in operator DNA recognition, is also critical for interaction with CarS, implying that the CarA-CarS protein-protein and the CarA-operator protein-DNA interfaces overlap. Thus, binding of CarA to operator and to antirepressor are mutually exclusive, and CarA may discern structural features in the acidic CarS protein that resemble operator DNA. Repressor inactivation by occluding the DNA-binding region may be a recurrent mechanism of action for acidic antirepressors.

PubMed: 17233828
DOI: 10.1111/j.1365-2958.2006.05567.x

実験手法

SOLUTION NMR