2JMC
Chimer between Spc-SH3 and P41
Summary for 2JMC
Entry DOI | 10.2210/pdb2jmc/pdb |
Related | 1TUC 2JMA |
NMR Information | BMRB: 15013 |
Descriptor | Spectrin alpha chain, brain and P41 peptide chimera (1 entity in total) |
Functional Keywords | chimer, spc-sh3, p41, signaling protein |
Biological source | Gallus gallus (chicken) |
Cellular location | Cytoplasm, cytoskeleton: P07751 |
Total number of polymer chains | 1 |
Total formula weight | 8462.51 |
Authors | van Nuland, N.A.J.,Candel, A.M.,Martinez, J.C.,Conejero-Lara, F.,Bruix, M. (deposition date: 2006-11-02, release date: 2007-04-24, Last modification date: 2024-11-20) |
Primary citation | Candel, A.M.,Conejero-Lara, F.,Martinez, J.C.,van Nuland, N.A.J.,Bruix, M. The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex Febs Lett., 581:687-692, 2007 Cited by PubMed Abstract: Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design. PubMed: 17275816DOI: 10.1016/j.febslet.2007.01.032 PDB entries with the same primary citation |
Experimental method | SOLUTION NMR |
Structure validation
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