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2JMC

Chimer between Spc-SH3 and P41

Summary for 2JMC
Entry DOI10.2210/pdb2jmc/pdb
Related1TUC 2JMA
NMR InformationBMRB: 15013
DescriptorSpectrin alpha chain, brain and P41 peptide chimera (1 entity in total)
Functional Keywordschimer, spc-sh3, p41, signaling protein
Biological sourceGallus gallus (chicken)
Cellular locationCytoplasm, cytoskeleton: P07751
Total number of polymer chains1
Total formula weight8462.51
Authors
van Nuland, N.A.J.,Candel, A.M.,Martinez, J.C.,Conejero-Lara, F.,Bruix, M. (deposition date: 2006-11-02, release date: 2007-04-24, Last modification date: 2024-11-20)
Primary citationCandel, A.M.,Conejero-Lara, F.,Martinez, J.C.,van Nuland, N.A.J.,Bruix, M.
The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex
Febs Lett., 581:687-692, 2007
Cited by
PubMed Abstract: Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.
PubMed: 17275816
DOI: 10.1016/j.febslet.2007.01.032
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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數據於2025-07-23公開中

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