2JMC

Chimer between Spc-SH3 and P41

2JMC の概要

• エントリーDOI: 10.2210/pdb2jmc/pdb
• 関連するPDBエントリー: 1TUC 2JMA
• NMR情報: BMRB: 15013
• 分子名称: Spectrin alpha chain, brain and P41 peptide chimera (1 entity in total)
• 機能のキーワード: chimer, spc-sh3, p41, signaling protein
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Cytoplasm, cytoskeleton: P07751
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8462.51

構造登録者

van Nuland, N.A.J.,Candel, A.M.,Martinez, J.C.,Conejero-Lara, F.,Bruix, M. (登録日: 2006-11-02, 公開日: 2007-04-24, 最終更新日: 2024-11-20)

主引用文献

Candel, A.M.,Conejero-Lara, F.,Martinez, J.C.,van Nuland, N.A.J.,Bruix, M.
The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex
Febs Lett., 581:687-692, 2007

PubMed Abstract: Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.

PubMed: 17275816
DOI: 10.1016/j.febslet.2007.01.032

実験手法

SOLUTION NMR