2JMA

R21A Spc-SH3:P41 complex

Summary for 2JMA

• Entry DOI: 10.2210/pdb2jma/pdb
• Related: 2jm8 2jm9
• NMR Information: BMRB: 7306
• Descriptor: Spectrin alpha chain, brain, P41 peptide (2 entities in total)
• Functional Keywords: sh3 domain, p41-bound, structural protein
• Biological source: Gallus gallus (chicken); More
• Cellular location: Cytoplasm, cytoskeleton: P07751
• Total number of polymer chains: 2
• Total formula weight: 8178.28

Authors

van Nuland, N.A.J.,Casares, S.,Ab, E.,Eshuis, H.,Lopez-Mayorga, O.,Conejero-Lara, F. (deposition date: 2006-10-25, release date: 2007-04-24, Last modification date: 2024-11-20)

Primary citation

Casares, S.,Ab, E.,Eshuis, H.,Lopez-Mayorga, O.,van Nuland, N.A.J.,Conejero-Lara, F.
The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: Understanding the determinants of binding affinity by comparison with Abl-SH3
Bmc Struct.Biol., 7:22-22, 2007

PubMed Abstract: SH3 domains are small protein modules of 60-85 amino acids that bind to short proline-rich sequences with moderate-to-low affinity and specificity. Interactions with SH3 domains play a crucial role in regulation of many cellular processes (some are related to cancer and AIDS) and have thus been interesting targets in drug design. The decapeptide APSYSPPPPP (p41) binds with relatively high affinity to the SH3 domain of the Abl tyrosine kinase (Abl-SH3), while it has a 100 times lower affinity for the alpha-spectrin SH3 domain (Spc-SH3).

PubMed: 17407569
DOI: 10.1186/1472-6807-7-22

Experimental method

SOLUTION NMR