2JM9

R21A Spc-SH3 bound

2JM9 の概要

• エントリーDOI: 10.2210/pdb2jm9/pdb
• 関連するPDBエントリー: 2jm8 2jmA
• NMR情報: BMRB: 7306
• 分子名称: Spectrin alpha chain, brain (1 entity in total)
• 機能のキーワード: sh3 domain, p41-bound, structural protein
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Cytoplasm, cytoskeleton: P07751
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7143.13

構造登録者

van Nuland, N.A.J.,Casares, S.,Ab, E.,Eshuis, H.,Lopez-Mayorga, O.,Conejero-Lara, F. (登録日: 2006-10-25, 公開日: 2007-04-24, 最終更新日: 2023-12-20)

主引用文献

Casares, S.,Ab, E.,Eshuis, H.,Lopez-Mayorga, O.,van Nuland, N.A.J.,Conejero-Lara, F.
The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: Understanding the determinants of binding affinity by comparison with Abl-SH3
Bmc Struct.Biol., 7:22-22, 2007

PubMed Abstract: SH3 domains are small protein modules of 60-85 amino acids that bind to short proline-rich sequences with moderate-to-low affinity and specificity. Interactions with SH3 domains play a crucial role in regulation of many cellular processes (some are related to cancer and AIDS) and have thus been interesting targets in drug design. The decapeptide APSYSPPPPP (p41) binds with relatively high affinity to the SH3 domain of the Abl tyrosine kinase (Abl-SH3), while it has a 100 times lower affinity for the alpha-spectrin SH3 domain (Spc-SH3).

PubMed: 17407569
DOI: 10.1186/1472-6807-7-22

実験手法

SOLUTION NMR