2JIB

X-ray structure of Oxalyl-CoA decarboxylase in complex with coenzyme- A

2JIB の概要

• エントリーDOI: 10.2210/pdb2jib/pdb
• 関連するPDBエントリー: 2C31 2JI6 2JI7 2JI8 2JI9
• 分子名称: OXALYL-COA DECARBOXYLASE, THIAMINE DIPHOSPHATE, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: lyase, flavoprotein, decarboxylase, thiamin diphosphate-dependent, substrate complex, oxalate degradation, thiamine pyrophosphate, non- oxidative decarboxylase
• 由来する生物種: OXALOBACTER FORMIGENES
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 125088.79

構造登録者

Berthold, C.L.,Toyota, C.G.,Moussatche, P.,Wood, M.D.,Leeper, F.,Richards, N.G.J.,Lindqvist, Y. (登録日: 2007-02-26, 公開日: 2007-07-17, 最終更新日: 2023-12-13)

主引用文献

Berthold, C.L.,Toyota, C.G.,Moussatche, P.,Wood, M.D.,Leeper, F.,Richards, N.G.J.,Lindqvist, Y.
Crystallographic Snapshots of Oxalyl-Coa Decarboxylase Give Insights Into Catalysis by Nonoxidative Thdp-Dependent Decarboxylases
Structure, 15:853-, 2007

PubMed Abstract: Despite more than five decades of extensive studies of thiamin diphosphate (ThDP) enzymes, there remain many uncertainties as to how these enzymes achieve their rate enhancements. Here, we present a clear picture of catalysis for the simple nonoxidative decarboxylase, oxalyl-coenzyme A (CoA) decarboxylase, based on crystallographic snapshots along the catalytic cycle and kinetic data on active site mutants. First, we provide crystallographic evidence that, upon binding of oxalyl-CoA, the C-terminal 13 residues fold over the substrate, aligning the substrate alpha-carbon for attack by the ThDP-C2 atom. The second structure presented shows a covalent reaction intermediate after decarboxylation, interpreted as being nonplanar. Finally, the structure of a product complex is presented. In accordance with mutagenesis data, no side chains of the enzyme are implied to directly participate in proton transfer except the glutamic acid (Glu-56), which promotes formation of the 1',4'-iminopyrimidine tautomer of ThDP needed for activation.

PubMed: 17637344
DOI: 10.1016/J.STR.2007.06.001

実験手法

X-RAY DIFFRACTION (2.2 Å)