2JHB

CORE BINDING FACTOR BETA

2JHB の概要

• エントリーDOI: 10.2210/pdb2jhb/pdb
• 分子名称: PROTEIN (CORE BINDING FACTOR BETA) (1 entity in total)
• 機能のキーワード: core binding factor, transcription factor, gene regulation
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16757.78

構造登録者

Huang, X.,Peng, J.,Speck, N.A.,Bushweller, J.H. (登録日: 1999-03-17, 公開日: 1999-07-05, 最終更新日: 2023-12-27)

主引用文献

Huang, X.,Peng, J.W.,Speck, N.A.,Bushweller, J.H.
Solution structure of core binding factor beta and map of the CBF alpha binding site.
Nat.Struct.Biol., 6:624-627, 1999

PubMed Abstract: The core binding factor beta subunit (CBF beta) is the non-DNA binding subunit of the core-binding factors, transcription factors essential for multiple developmental processes including hematopoiesis and bone development. Chromosomal translocations involving the human CBFB gene are associated with a large percentage of human leukemias. The N-terminal 141 amino acids of CBF beta contains the heterodimerization domain for the DNA-binding CBF alpha subunits, and is sufficient for CBF beta function in vivo. Here we present the high-resolution solution structure of the CBF beta heterodimerization domain. It is a novel alpha/beta structure consisting of two three-stranded beta-sheets packed on one another in a sandwich arrangement, with four peripheral alpha-helices. The CBF alpha binding site on CBF beta has been mapped by chemical shift perturbation analysis.

PubMed: 10404216
DOI: 10.1038/10670

実験手法

SOLUTION NMR