2JH3
The crystal structure of DR2241 from Deinococcus radiodurans at 1.9 A resolution reveals a multi-domain protein with structural similarity to chelatases but also with two additional novel domains
Summary for 2JH3
| Entry DOI | 10.2210/pdb2jh3/pdb |
| Descriptor | RIBOSOMAL PROTEIN S2-RELATED PROTEIN, IRON/SULFUR CLUSTER (3 entities in total) |
| Functional Keywords | cbix, sad phasing, ribosomal protein, structural genomics, chelatase super-family fold, 4fe-4s iron-sulphur cluster |
| Biological source | DEINOCOCCUS RADIODURANS |
| Total number of polymer chains | 4 |
| Total formula weight | 205681.01 |
| Authors | Leiros, H.-K.S.,Mcsweeney, S.M. (deposition date: 2007-02-20, release date: 2007-06-19, Last modification date: 2024-05-08) |
| Primary citation | Leiros, H.-K.S.,Mcsweeney, S.M. The Crystal Structure of Dr2241 from Deinococcus Radiodurans at 1.9 A Resolution Reveals a Multi-Domain Protein with Structural Similarity to Chelatases But Also with Two Additional Novel Domains J.Struct.Biol., 159:92-, 2007 Cited by PubMed Abstract: A unique family of proteins have been identified in the Deinococcus genus with an N-terminal cobalamin (vitamin B(12)) chelatase domain denoted CbiX and an additional unique C-terminal domain with unknown function. Here we report the first crystal structure from this new family of proteins with the structure of Deinococcus radiodurans protein DR2241. The structure reveals a multi-domain protein where domains A (residues 1-132) has the same fold as the small CbiX (CbiX(S)), domains A and B (residues 1-272) follow the chelatase super-family fold and the two additional unique domains C and D have no structural homologues. Domain D harbours the sequence motifs CxxC and CxxxC, in which DR2241 gives the first evidence that these motifs bind a [4Fe-4S] iron-sulphur cluster. In solution there are indications of multimeric forms, and in the crystallographic asymmetric unit a tetramer is found where domains C and D are involved in stabilising the tetrameric assembly. PubMed: 17448684DOI: 10.1016/J.JSB.2007.02.009 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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