2JEX

Transcription activator structure reveals redox control of a replication initiation reaction

2JEX の概要

• エントリーDOI: 10.2210/pdb2jex/pdb
• 関連するPDBエントリー: 1DBD 1JJH 2BOP 2JEU
• 分子名称: REGULATORY PROTEIN E2 (2 entities in total)
• 機能のキーワード: nuclear protein, dna replication, phosphorylation, transcription regulation, viral transcription factor, bovine papillomavirus, replication initiation, early protein, transcription, redox control, e1, e2, oxidation, activator, repressor, dna-binding
• 由来する生物種: BOVINE PAPILLOMAVIRUS TYPE 1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23820.59

構造登録者

Sanders, C.M.,Sizov, D.,Seavers, P.R.,Ortiz-Lombardia, M.,Antson, A.A. (登録日: 2007-01-24, 公開日: 2007-05-15, 最終更新日: 2023-12-13)

主引用文献

Sanders, C.M.,Sizov, D.,Seavers, P.R.,Ortiz-Lombardia, M.,Antson, A.A.
Transcription Activator Structure Reveals Redox Control of a Replication Initiation Reaction.
Nucleic Acids Res., 35:3504-, 2007

PubMed Abstract: Redox changes are one of the factors that influence cell-cycle progression and that control the processes of cellular proliferation, differentiation, senescence and apoptosis. Proteins regulated through redox-sensitive cysteines have been characterized but specific 'sulphydryl switches' in replication proteins remain to be identified. In bovine papillomavirus type-1, DNA replication begins when the viral transcription factor E2 recruits the viral initiator protein E1 to the origin of DNA replication (ori). Here we show that a novel dimerization interface in the E2 transcription activation domain is stabilized by a disulphide bond. Oxidative cross-linking via Cys57 sequesters the interaction surface between E1 and E2, preventing pre-initiation and replication initiation complex formation. Our data demonstrate that as well as a mechanism for regulating DNA binding, redox reactions can control replication by modulating the tertiary structure of critical protein factors using a specific redox sensor.

PubMed: 17478495
DOI: 10.1093/NAR/GKM166

実験手法

X-RAY DIFFRACTION (2.35 Å)