2JDF

Human gamma-B crystallin

2JDF の概要

• エントリーDOI: 10.2210/pdb2jdf/pdb
• 関連するPDBエントリー: 1LEU 1MYV 1MYX 1MYY 1MZ1 1MZ2 1MZ3 2JDG
• 分子名称: GAMMA CRYSTALLIN B (2 entities in total)
• 機能のキーワード: artificial binding protein, structural protein, eye lens protein, gamma crystallin, affilin, oxidation, polymorphism, phosphorylation
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21873.63

構造登録者

Ebersbach, H.,Fiedler, E.,Scheuermann, T.,Fiedler, M.,Stubbs, M.T.,Reimann, C.,Proetzel, G.,Rudolph, R.,Fiedler, U. (登録日: 2007-01-08, 公開日: 2007-07-24, 最終更新日: 2024-05-08)

主引用文献

Ebersbach, H.,Fiedler, E.,Scheuermann, T.,Fiedler, M.,Stubbs, M.T.,Reimann, C.,Proetzel, G.,Rudolph, R.,Fiedler, U.
Affilin-Novel Binding Molecules Based on Human Gamma-B-Crystallin, an All Beta-Sheet Protein.
J.Mol.Biol., 372:172-, 2007

PubMed Abstract: The concept of novel binding proteins as an alternative to antibodies has undergone rapid development and is now ready for practical use in a wide range of applications. Alternative binding proteins, based on suitable scaffolds with desirable properties, are selected from combinatorial libraries in vitro. Here, we describe an approach using a beta-sheet of human gamma-B-crystallin to generate a universal binding site through randomization of eight solvent-exposed amino acid residues selected according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against a variety of targets that differ considerably in size and structure. The isolated Affilin variants can be produced in Escherichia coli as soluble proteins and have a high level of thermodynamic stability. The crystal structures of the human wild-type gamma-B-crystallin and a selected Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human gamma-B-crystallin tolerates amino acid exchanges with no major structural change. We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules.

PubMed: 17628592
DOI: 10.1016/J.JMB.2007.06.045

実験手法

X-RAY DIFFRACTION (1.7 Å)