2JCP

The hyaluronan binding domain of murine CD44

Summary for 2JCP

• Entry DOI: 10.2210/pdb2jcp/pdb
• Related: 2JCQ 2JCR
• Descriptor: CD44 ANTIGEN (2 entities in total)
• Functional Keywords: sugar-binding protein, hyauronan, link-domain, proteoglycan, polymorphism, blood group antigen, alternative splicing, lectin, antigen, membrane, receptor, sulfation, glycoprotein, c-type lectin, cell adhesion, extracellular matrix, pyrrolidone carboxylic acid, transmembrane, phosphorylation, sugar binding protein
• Biological source: MUS MUSCULUS (MOUSE)
• Total number of polymer chains: 1
• Total formula weight: 17143.07

Authors

Banerji, S.,Wright, A.J.,Noble, M.E.M.,Mahoney, D.J.,Campbell, I.D.,Day, A.J.,Jackson, D.G. (deposition date: 2007-01-03, release date: 2007-01-30, Last modification date: 2024-02-07)

Primary citation

Banerji, S.,Wright, A.J.,Noble, M.E.M.,Mahoney, D.J.,Campbell, I.D.,Day, A.J.,Jackson, D.G.
Structures of the Cd44-Hyaluronan Complex Provide Insight Into a Fundamental Carbohydrate-Protein Interaction.
Nat.Struct.Mol.Biol., 14:234-, 2008

PubMed Abstract: Regulation of transient interactions between cells and the ubiquitous matrix glycosaminoglycan hyaluronan is crucial to such fundamental processes as embryonic development and leukocyte homing. Cd44, the primary cell surface receptor for hyaluronan, binds ligand via a lectin-like fold termed the Link module, but only after appropriate functional activation. The molecular details of the Cd44-hyaluronan interaction and hence the structural basis for this activation are unknown. Here we present the first crystal structure of Cd44 complexed with hyaluronan. This reveals that the interaction with hyaluronan is dominated by shape and hydrogen-bonding complementarity and identifies two conformational forms of the receptor that differ in orientation of a crucial hyaluronan-binding residue (Arg45, equivalent to Arg41 in human CD44). Measurements by NMR indicate that the conformational transition can be induced by hyaluronan binding, providing further insight into possible mechanisms for regulation of Cd44.

PubMed: 17293874
DOI: 10.1038/NSMB1201

Experimental method

X-RAY DIFFRACTION (1.3 Å)