2JCP

The hyaluronan binding domain of murine CD44

2JCP の概要

• エントリーDOI: 10.2210/pdb2jcp/pdb
• 関連するPDBエントリー: 2JCQ 2JCR
• 分子名称: CD44 ANTIGEN (2 entities in total)
• 機能のキーワード: sugar-binding protein, hyauronan, link-domain, proteoglycan, polymorphism, blood group antigen, alternative splicing, lectin, antigen, membrane, receptor, sulfation, glycoprotein, c-type lectin, cell adhesion, extracellular matrix, pyrrolidone carboxylic acid, transmembrane, phosphorylation, sugar binding protein
• 由来する生物種: MUS MUSCULUS (MOUSE)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17143.07

構造登録者

Banerji, S.,Wright, A.J.,Noble, M.E.M.,Mahoney, D.J.,Campbell, I.D.,Day, A.J.,Jackson, D.G. (登録日: 2007-01-03, 公開日: 2007-01-30, 最終更新日: 2024-11-13)

主引用文献

Banerji, S.,Wright, A.J.,Noble, M.E.M.,Mahoney, D.J.,Campbell, I.D.,Day, A.J.,Jackson, D.G.
Structures of the Cd44-Hyaluronan Complex Provide Insight Into a Fundamental Carbohydrate-Protein Interaction.
Nat.Struct.Mol.Biol., 14:234-, 2008

PubMed Abstract: Regulation of transient interactions between cells and the ubiquitous matrix glycosaminoglycan hyaluronan is crucial to such fundamental processes as embryonic development and leukocyte homing. Cd44, the primary cell surface receptor for hyaluronan, binds ligand via a lectin-like fold termed the Link module, but only after appropriate functional activation. The molecular details of the Cd44-hyaluronan interaction and hence the structural basis for this activation are unknown. Here we present the first crystal structure of Cd44 complexed with hyaluronan. This reveals that the interaction with hyaluronan is dominated by shape and hydrogen-bonding complementarity and identifies two conformational forms of the receptor that differ in orientation of a crucial hyaluronan-binding residue (Arg45, equivalent to Arg41 in human CD44). Measurements by NMR indicate that the conformational transition can be induced by hyaluronan binding, providing further insight into possible mechanisms for regulation of Cd44.

PubMed: 17293874
DOI: 10.1038/NSMB1201

実験手法

X-RAY DIFFRACTION (1.3 Å)