2J8I

Structure of NP275, a pentapeptide repeat protein from Nostoc punctiforme

2J8I の概要

• エントリーDOI: 10.2210/pdb2j8i/pdb
• 関連するPDBエントリー: 2J8K
• 分子名称: NP275, CHLORIDE ION (3 entities in total)
• 機能のキーワード: toxin
• 由来する生物種: NOSTOC PUNCTIFORME
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25029.25

構造登録者

Vetting, M.W.,Hegde, S.S.,Hazleton, K.Z.,Blanchard, J.S. (登録日: 2006-10-25, 公開日: 2006-12-07, 最終更新日: 2023-12-13)

主引用文献

Vetting, M.W.,Hegde, S.S.,Hazleton, K.Z.,Blanchard, J.S.
Structural Characterization of the Fusion of Two Pentapeptide Repeat Proteins, Np275 and Np276, from Nostoc Punctiforme: Resurrection of an Ancestral Protein.
Protein Sci., 16:755-, 2007

PubMed Abstract: The Nostoc punctiforme genes Np275 and Np276 are two adjacently encoded proteins of 98 and 75 amino acids in length and exhibit sequences composed of tandem pentapeptide repeats. The structures of Np275 and a fusion of Np275 and Np276 were determined to 2.1 and 1.5 A, respectively. The two Nostoc proteins fold as highly symmetric right-handed quadrilateral beta-helices similar to the mycobacterial protein MfpA implicated in fluoroquinolone resistance and DNA gyrase inhibition. The sequence composition of the intervening coding region and the ability to express a fused protein by removing the stop codon for Np275 suggests Np275 and Np276 were recently part of a larger ancestral pentapeptide repeat protein.

PubMed: 17384236
DOI: 10.1110/PS.062637707

実験手法

X-RAY DIFFRACTION (2.1 Å)