2J76

Solution structure and RNA interactions of the RNA recognition motif from eukaryotic translation initiation factor 4B

2J76 の概要

• エントリーDOI: 10.2210/pdb2j76/pdb
• 関連するPDBエントリー: 1WI8
• 分子名称: EUKARYOTIC TRANSLATION INITIATION FACTOR 4B (1 entity in total)
• 機能のキーワード: protein biosynthesis, rna recognition motif, initiation factor, rna binding domain, translation, rrm, rbd, rnp, rna-binding, phosphorylation
• 由来する生物種: HOMO SAPIENS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11457.73

構造登録者

Fleming, K.,Ghuman, J.,Yuan, X.M.,Simpson, P.,Szendroi, A.,Matthews, S.,Curry, S. (登録日: 2006-10-06, 公開日: 2008-10-28, 最終更新日: 2024-05-15)

主引用文献

Fleming, K.,Ghuman, J.,Yuan, X.M.,Simpson, P.,Szendroi, A.,Matthews, S.,Curry, S.
Solution Structure and RNA Interactions of the RNA Recognition Motif from Eukaryotic Translation Initiation Factor 4B.
Biochemistry, 42:8966-, 2003

PubMed Abstract: Eukaryotic initiation factor 4B (eIF4B) is a multidomain protein with a range of activities that serves primarily to promote association of messenger RNA to the 40S ribosomal subunit during translation initiation. We report here the solution structure of the eIF4B RNA recognition motif (RRM) domain. It adopts a classical RRM fold, with a beta alpha beta beta alpha beta topology. The most striking difference with other RRM structures is in the disposition of loop 3, which connects the beta 2 and beta 3 strands and is implicated in RNA recognition. This loop folds down against the body of the RRM and exhibits restricted motion on a milli- to microsecond time scale. Although it contributes to a large basic patch on the RNA binding surface, it does not protrude out from the domain as observed in other RRM structures, possibly implying a different mode of RNA binding. On its own, the core RRM domain provides only a relative weak interaction with RNA targets and appears to require extensions at the N- and C-terminus for high-affinity binding.

PubMed: 12885229
DOI: 10.1021/BI034506G

実験手法

SOLUTION NMR