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2J74

Structure of Beta-1,4-Galactanase

Summary for 2J74
Entry DOI10.2210/pdb2j74/pdb
Related1UR0 1UR4 2CCR
Related PRD IDPRD_900113 PRD_900114
DescriptorYVFO, beta-D-galactopyranose-(1-4)-beta-D-galactopyranose-(1-4)-beta-D-galactopyranose, beta-D-galactopyranose-(1-4)-beta-D-galactopyranose, ... (5 entities in total)
Functional Keywordsglycoside hydrolase family 53, hydrolase, gh-a, beta-1, galactan, 4-galactanase
Biological sourceBACILLUS LICHENIFORMIS
Total number of polymer chains2
Total formula weight89330.49
Authors
Le Nours, J.,De Maria, L.,Welner, D.,Jorgensen, C.T.,Christensen, L.L.H.,Larsen, S.,Lo Leggio, L. (deposition date: 2006-10-06, release date: 2007-12-18, Last modification date: 2023-12-13)
Primary citationLe Nours, J.,De Maria, L.,Welner, D.,Jorgensen, C.T.,Christensen, L.L.H.,Borchert, T.V.,Larsen, S.,Lo Leggio, L.
Investigating the Binding of Beta-1,4-Galactan to Bacillus Licheniformis Beta-1,4-Galactanase by Crystallography and Computational Modeling.
Proteins, 75:977-, 2009
Cited by
PubMed Abstract: Microbial beta-1,4-galactanases are glycoside hydrolases belonging to family 53, which degrade galactan and arabinogalactan side chains in the hairy regions of pectin, a major plant cell wall component. They belong to the larger clan GH-A of glycoside hydrolases, which cover many different poly- and oligosaccharidase specificities. Crystallographic complexes of Bacillus licheniformis beta-1,4-galactanase and its inactive nucleophile mutant have been obtained with methyl-beta(1-->4)-galactotetraoside, providing, for the first time, information on substrate binding to the aglycone side of the beta-1,4-galactanase substrate binding groove. Using the experimentally determined subsites as a starting point, a beta(1-->4)-galactononaose was built into the structure and subjected to molecular dynamics simulations giving further insight into the residues involved in the binding of the polysaccharide from subsite -4 to +5. In particular, this analysis newly identified a conserved beta-turn, which contributes to subsites -2 to +3. This beta-turn is unique to family 53 beta-1,4-galactanases among all clan GH-A families that have been structurally characterized and thus might be a structural signature for endo-beta-1,4-galactanase specificity.
PubMed: 19089956
DOI: 10.1002/PROT.22310
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.6 Å)
Structure validation

229380

数据于2024-12-25公开中

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