2J6X

The crystal structure of lactate oxidase

2J6X の概要

• エントリーDOI: 10.2210/pdb2j6x/pdb
• 分子名称: LACTATE OXIDASE, FLAVIN MONONUCLEOTIDE, ZINC ION, ... (4 entities in total)
• 機能のキーワード: biosensors, flavoenzyme, fmn-dependent, oxidoreductase
• 由来する生物種: AEROCOCCUS VIRIDANS
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 330986.29

構造登録者

Leiros, I.,Wang, E.,Rasmussen, T.,Oksanen, E.,Repo, H.,Petersen, S.B.,Heikinheimo, P.,Hough, E. (登録日: 2006-10-05, 公開日: 2006-10-23, 最終更新日: 2023-12-13)

主引用文献

Leiros, I.,Wang, E.,Rasmussen, T.,Oksanen, E.,Repo, H.,Petersen, S.B.,Heikinheimo, P.,Hough, E.
The 2.1 A Structure of Aerococcus Viridans L-Lactate Oxidase (Lox).
Acta Crystallogr.,Sect.F, 62:1185-, 2006

PubMed Abstract: The crystal structure of L-lactate oxidase (LOX) from Aerococcus viridans has been determined at 2.1 A resolution. LOX catalyzes the flavin mononucleotide (FMN) dependent oxidation of lactate to pyruvate and hydrogen peroxide. LOX belongs to the alpha-hydroxy-acid oxidase flavoenzyme family; members of which bind similar substrates and to some extent have conserved catalytic properties and structural motifs. LOX crystallized as two tightly packed tetramers in the asymmetric unit, each having fourfold symmetry. The present structure shows a conserved FMN coordination, but also reveals novel residues involved in substrate binding compared with other family members.

PubMed: 17142893
DOI: 10.1107/S1744309106044678

実験手法

X-RAY DIFFRACTION (2.1 Å)