2J68

Bacterial dynamin-like protein BDLP, GDP bound

2J68 の概要

• エントリーDOI: 10.2210/pdb2j68/pdb
• 関連するPDBエントリー: 2J69
• 分子名称: BACTERIAL DYNAMIN-LIKE PROTEIN, GUANOSINE-5'-DIPHOSPHATE (2 entities in total)
• 機能のキーワード: dynamin, fzo, fzl, gtpase, hydrolase
• 由来する生物種: NOSTOC PUNCTIFORME
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 79161.18

構造登録者

Low, H.H.,Lowe, J. (登録日: 2006-09-26, 公開日: 2006-12-04, 最終更新日: 2023-12-13)

主引用文献

Low, H.H.,Lowe, J.
A Bacterial Dynamin-Like Protein
Nature, 444:766-, 2006

PubMed Abstract: Dynamins form a superfamily of large mechano-chemical GTPases that includes the classical dynamins and dynamin-like proteins (DLPs). They are found throughout the Eukarya, functioning in core cellular processes such as endocytosis and organelle division. Many bacteria are predicted by sequence to possess large GTPases with the same multidomain architecture that is found in DLPs. Mechanistic dissection of dynamin family members has been impeded by a lack of high-resolution structural data currently restricted to the GTPase and pleckstrin homology domains, and the dynamin-related human guanylate-binding protein. Here we present the crystal structure of a cyanobacterial DLP in both nucleotide-free and GDP-associated conformation. The bacterial DLP shows dynamin-like qualities, such as helical self-assembly and tubulation of a lipid bilayer. In vivo, it localizes to the membrane in a manner reminiscent of FZL, a chloroplast-specific dynamin-related protein with which it shares sequence similarity. Our results provide structural and mechanistic insight that may be relevant across the dynamin superfamily. Concurrently, we show compelling similarity between a cyanobacterial and chloroplast DLP that, given the endosymbiotic ancestry of chloroplasts, questions the evolutionary origins of dynamins.

PubMed: 17122778
DOI: 10.1038/NATURE05312

実験手法

X-RAY DIFFRACTION (3.1 Å)