2J5P

E. coli FtsK gamma domain

2J5P の概要

• エントリーDOI: 10.2210/pdb2j5p/pdb
• 関連するPDBエントリー: 2IUS 2J5O
• 分子名称: DNA TRANSLOCASE FTSK (1 entity in total)
• 機能のキーワード: ftsk, kops, xercd, recombination, dna segregation, bacterial cell division
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cell inner membrane ; Multi-pass membrane protein : P46889
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8680.66

構造登録者

Sivanathan, V.,Allen, M.D.,de Bekker, C.,Baker, R.,Arciszewska, L.,Freund, S.M.,Bycroft, M.,Lowe, J.,Sherratt, D.J. (登録日: 2006-09-19, 公開日: 2006-10-04, 最終更新日: 2024-05-15)

主引用文献

Sivanathan, V.,Allen, M.D.,De Bekker, C.,Baker, R.,Arciszewska, L.,Freund, S.M.,Bycroft, M.,Lowe, J.,Sherratt, D.J.
The Ftsk Gamma Domain Directs Oriented DNA Translocation by Interacting with Kops.
Nat.Struct.Mol.Biol., 13:965-, 2006

PubMed Abstract: The bacterial septum-located DNA translocase FtsK coordinates circular chromosome segregation with cell division. Rapid translocation of DNA by FtsK is directed by 8-base-pair DNA motifs (KOPS), so that newly replicated termini are brought together at the developing septum, thereby facilitating completion of chromosome segregation. Translocase functions reside in three domains, alpha, beta and gamma. FtsKalphabeta are necessary and sufficient for ATP hydrolysis-dependent DNA translocation, which is modulated by FtsKgamma through its interaction with KOPS. By solving the FtsKgamma structure by NMR, we show that gamma is a winged-helix domain. NMR chemical shift mapping localizes the DNA-binding site on the gamma domain. Mutated proteins with substitutions in the FtsKgamma DNA-recognition helix are impaired in DNA binding and KOPS recognition, yet remain competent in DNA translocation and XerCD-dif site-specific recombination, which facilitates the late stages of chromosome segregation.

PubMed: 17057717
DOI: 10.1038/NSMB1158

実験手法

SOLUTION NMR