2J4R

Structural Study of the Aquifex aeolicus PPX-GPPA enzyme

2J4R の概要

• エントリーDOI: 10.2210/pdb2j4r/pdb
• 関連するPDBエントリー: 1T6C 1T6D
• 分子名称: EXOPOLYPHOSPHATASE, GUANOSINE-5',3'-TETRAPHOSPHATE (3 entities in total)
• 機能のキーワード: ppgpp, aquifex aeolicus, stringent response, hydrolase, exopolyphosphatase, guanosine pentaphosphate
• 由来する生物種: AQUIFEX AEOLICUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 70600.03

構造登録者

Kristensen, O. (登録日: 2006-09-05, 公開日: 2007-10-16, 最終更新日: 2023-12-13)

主引用文献

Kristensen, O.,Ross, B.,Gajhede, M.
Structure of the Ppx/Gppa Phosphatase from Aquifex Aeolicus in Complex with the Alarmone Ppgpp
J.Mol.Biol., 375:1469-, 2008

PubMed Abstract: The crystal structure of the prototype exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family member from Aquifex aeolicus in complex with the intracellular second messenger guanosine tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is identified as E119. The dual specificity established for the Escherichia coli homolog is shown to be compatible with a common active site for guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and different degrees of closure between the two domains of the enzyme are associated with substrate binding. The arginines R22 and R267, residing in different domains, are crucial for guanosine pentaphosphate specificity as they interact with the unique 3'-ribose phosphorylation.

PubMed: 18155044
DOI: 10.1016/J.JMB.2007.11.073

実験手法

X-RAY DIFFRACTION (2.71 Å)