2J4N

Double dockerin from Piromyces equi Cel45A

Summary for 2J4N

• Entry DOI: 10.2210/pdb2j4n/pdb
• Related: 1E8P 1E8Q 2J4M
• NMR Information: BMRB: 7285
• Descriptor: ENDOGLUCANASE 45A (1 entity in total)
• Functional Keywords: dockerin, cellulosome, protein binding, small cysteine-rich
• Biological source: PIROMYCES EQUI
• Total number of polymer chains: 1
• Total formula weight: 10910.52

Authors

Nagy, T.,Tunnicliffe, R.B.,Higgins, L.D.,Walters, C.,Gilbert, H.J.,Williamson, M.P. (deposition date: 2006-09-01, release date: 2007-09-25, Last modification date: 2024-10-23)

Primary citation

Nagy, T.,Tunnicliffe, R.B.,Higgins, L.D.,Walters, C.,Gilbert, H.J.,Williamson, M.P.
Characterization of a Double Dockerin from the Cellulosome of the Anaerobic Fungus Piromyces Equi.
J.Mol.Biol., 373:612-, 2007

PubMed Abstract: The assembly into supramolecular complexes of proteins having complementary activities is central to cellular function. One such complex of considerable biological and industrial significance is the plant cell wall-degrading apparatus of anaerobic microorganisms, termed the cellulosome. A central feature of bacterial cellulosomes is a large non-catalytic protein, the scaffoldin, which contains multiple cohesin domains. An array of digestive enzymes is incorporated into the cellulosome through the interaction of the dockerin domains, present in the catalytic subunits, with the cohesin domains that are present in the scaffoldin. By contrast, in anaerobic fungi, such as Piromyces equi, the dockerins of cellulosomal enzymes are often present in tandem copies; however, the identity of the cognate cohesin domains in these organisms is unclear, hindering further biotechnological development of the fungal cellulosome. Here, we characterise the solution structure and function of a double-dockerin construct from the P. equi endoglucanase Cel45A. We show that the two domains are connected by a flexible linker that is short enough to keep the binding sites of the two domains on adjacent surfaces, and allows the double-dockerin construct to bind more tightly to cellulosomes than a single domain and with greater coverage. The double dockerin binds to the GH3 beta-glucosidase component of the fungal cellulosome, which is thereby identified as a potential scaffoldin.

PubMed: 17869267
DOI: 10.1016/J.JMB.2007.08.007

Experimental method

SOLUTION NMR