2J44

Alpha-glucan binding by a streptococcal virulence factor

2J44 の概要

• エントリーDOI: 10.2210/pdb2j44/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900009 PRD_900010
• 分子名称: ALKALINE AMYLOPULLULANASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: virulence, pullulanase, glycogen binding, streptococcus pneumoniae, carbohydrate-binding module
• 由来する生物種: STREPTOCOCCUS PNEUMONIAE
• 細胞内の位置: Secreted, cell wall; Peptidoglycan-anchor (By similarity): Q97SQ7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26719.33

構造登録者

Lammerts van Bueren, A.,Higgins, M.,Wang, D.,Burke, R.D.,Boraston, A.B. (登録日: 2006-08-24, 公開日: 2006-12-11, 最終更新日: 2023-12-13)

主引用文献

Lammerts Van Bueren, A.,Higgins, M.,Wang, D.,Burke, R.D.,Boraston, A.B.
Identification and Structural Basis of Binding to Host Lung Glycogen by Streptococcal Virulence Factors.
Nat.Struct.Mol.Biol., 14:76-, 2007

PubMed Abstract: The ability of pathogenic bacteria to recognize host glycans is often essential to their virulence. Here we report structure-function studies of previously uncharacterized glycogen-binding modules in the surface-anchored pullulanases from Streptococcus pneumoniae (SpuA) and Streptococcus pyogenes (PulA). Multivalent binding to glycogen leads to a strong interaction with alveolar type II cells in mouse lung tissue. X-ray crystal structures of the binding modules reveal a novel fusion of tandem modules into single, bivalent functional domains. In addition to indicating a structural basis for multivalent attachment, the structure of the SpuA modules in complex with carbohydrate provides insight into the molecular basis for glycogen specificity. This report provides the first evidence that intracellular lung glycogen may be a novel target of pathogenic streptococci and thus provides a rationale for the identification of the streptococcal alpha-glucan-metabolizing machinery as virulence factors.

PubMed: 17187076
DOI: 10.1038/NSMB1187

実験手法

X-RAY DIFFRACTION (2.1 Å)