2J3W

The crystal structure of the bet3-trs31-sedlin complex.

2J3W の概要

• エントリーDOI: 10.2210/pdb2j3w/pdb
• 関連するPDBエントリー: 2J3R 2J3T
• 分子名称: TRAFFICKING PROTEIN PARTICLE COMPLEX PROTEIN 2, ZGC 92866, TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3, ... (5 entities in total)
• 機能のキーワード: multisubunit tethering factor, trapp, palmitate, transport, lipoprotein, er-golgi transport, endoplasmic reticulum, transcription, golgi apparatus, vesicle transport
• 由来する生物種: MUS MUSCULUS (HOUSE MOUSE); 詳細
• 細胞内の位置: Golgi apparatus (By similarity): Q9CQP2; Golgi apparatus, cis-Golgi network (By similarity): 2J3W
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 116946.09

構造登録者

Kim, Y.-G.,Oh, B.-H. (登録日: 2006-08-23, 公開日: 2006-11-27, 最終更新日: 2024-10-23)

主引用文献

Kim, Y.-G.,Raunser, S.,Munger, C.,Wagner, J.,Song, Y.-L.,Cygler, M.,Walz, T.,Oh, B.-H.,Sacher, M.
The Architecture of the Multisubunit Trapp I Complex Suggests a Model for Vesicle Tethering.
Cell(Cambridge,Mass.), 127:817-, 2006

PubMed Abstract: Transport protein particle (TRAPP) I is a multisubunit vesicle tethering factor composed of seven subunits involved in ER-to-Golgi trafficking. The functional mechanism of the complex and how the subunits interact to form a functional unit are unknown. Here, we have used a multidisciplinary approach that includes X-ray crystallography, electron microscopy, biochemistry, and yeast genetics to elucidate the architecture of TRAPP I. The complex is organized through lateral juxtaposition of the subunits into a flat and elongated particle. We have also localized the site of guanine nucleotide exchange activity to a highly conserved surface encompassing several subunits. We propose that TRAPP I attaches to Golgi membranes with its large flat surface containing many highly conserved residues and forms a platform for protein-protein interactions. This study provides the most comprehensive view of a multisubunit vesicle tethering complex to date, based on which a model for the function of this complex, involving Rab1-GTP and long, coiled-coil tethers, is presented.

PubMed: 17110339
DOI: 10.1016/J.CELL.2006.09.029

実験手法

X-RAY DIFFRACTION (2.1 Å)