2J1V

Structure of a Streptococcus pneumoniae fucose binding module in complex with the blood group H-trisaccharide

Summary for 2J1V

• Entry DOI: 10.2210/pdb2j1v/pdb
• Related: 2J1R 2J1S 2J1T 2J1U 2J22
• Related PRD ID: PRD_900036
• Descriptor: FUCOLECTIN-RELATED PROTEIN, alpha-L-fucopyranose-(1-2)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: carbohydrate-binding protein, carbohydrate binding protein
• Biological source: STREPTOCOCCUS PNEUMONIAE
• Total number of polymer chains: 2
• Total formula weight: 34300.86

Authors

Boraston, A.B.,Wang, D.,Burke, R.D. (deposition date: 2006-08-15, release date: 2006-09-06, Last modification date: 2024-05-08)

Primary citation

Boraston, A.B.,Wang, D.,Burke, R.D.
Blood Group Antigen Recognition by a Streptococcus Pneumoniae Virulence Factor
J.Biol.Chem., 281:35263-, 2006

PubMed Abstract: The Streptococcus pneumoniae fucose utilization operon includes a gene encoding a virulence factor that belongs to family 98 in the glycoside hydrolase classification. This protein contains a C-terminal triplet of fucose binding modules that have significant amino acid sequence identity with the Anguilla anguilla fucolectin. Functional studies of these fucose binding modules reveal binding to fucosylated oligosaccharides and suggest the importance of multivalent binding. The high resolution crystal structures of ligand bound forms of one fucose binding module uncovers the molecular basis of fucose, ABH blood group antigen, and Lewisy antigen binding. These studies are extended by fluorescence microscopy to show specific binding to mouse lung tissue. These modules define a new family of carbohydrate binding modules now classified as family 47.

PubMed: 16987809
DOI: 10.1074/JBC.M607620200

Experimental method

X-RAY DIFFRACTION (1.45 Å)