2J0B

Structure of the catalytic domain of mouse Manic Fringe in complex with UDP and manganese

2J0B の概要

• エントリーDOI: 10.2210/pdb2j0b/pdb
• 関連するPDBエントリー: 2J0A
• 分子名称: BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE MANIC FRINGE, SULFATE ION, POTASSIUM ION, ... (6 entities in total)
• 機能のキーワード: glycosyltransferase, developmental protein, transmembrane, golgi apparatus, notch signaling, membrane, transferase, glycoprotein, signal-anchor
• 由来する生物種: MUS MUSCULUS (MOUSE)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32143.55

構造登録者

Jinek, M.,Chen, Y.-W.,Clausen, H.,Cohen, S.M.,Conti, E. (登録日: 2006-08-01, 公開日: 2006-09-04, 最終更新日: 2024-10-23)

主引用文献

Jinek, M.,Chen, Y.-W.,Clausen, H.,Cohen, S.M.,Conti, E.
Structural Insights Into the Notch-Modifying Glycosyltransferase Fringe
Nat.Struct.Mol.Biol., 13:945-, 2006

PubMed Abstract: Fringe proteins are beta1,3-N-acetylglucosaminyltransferases that modify Notch receptors, altering their ligand-binding specificity to regulate Notch signaling in development. We present the crystal structure of mouse Manic Fringe bound to UDP and manganese. The structure reveals amino acid residues involved in recognition of donor substrates and catalysis, and a putative binding pocket for acceptor substrates. Mutations of several invariant residues in this pocket impair Fringe activity in vivo.

PubMed: 16964258
DOI: 10.1038/NSMB1144

実験手法

X-RAY DIFFRACTION (2.1 Å)